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5) Use specific primers: If you have specific primer sequences for its region, you can search for these primers in the sequencing results to locate its region. 6) Visual alignment: Use sequence alignment tools (such as clustal omega, mafft) to compare the sequencing results. Fungi (its) and protists (18s) are classified for different categories of microbial diversity. Their amplification regions are different, and the corresponding amplification primers are also different. It mainly depends on what kind of microbial diversity information you want to obtain. There is no difference in anything else, sequencing.
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